Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes
نویسندگان
چکیده
منابع مشابه
High-Throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire
Currently existing immune repertoire sequencing technologies yield data on only one of the two chains of immune receptors [1–3]. Sequence analysis of VH:VL pairs is therefore currently performed by microtiter-well sorting of individual B cells followed by single-cell RT-PCR (scRT-PCR) and Sanger sequencing [3–10]; however at most a few hundred VH:VL pairs (a number dwarfed by the enormous size ...
متن کاملHigh-Throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire
Currently existing immune repertoire sequencing technologies yield data on only one of the two chains of immune receptors [1–3]. Sequence analysis of VH:VL pairs is therefore currently performed by microtiter-well sorting of individual B cells followed by single-cell RT-PCR (scRT-PCR) and Sanger sequencing [3–10]; however at most a few hundred VH:VL pairs (a number dwarfed by the enormous size ...
متن کاملHeavy and Light Chain Allotypic Markers on Rabbit Homocytotropic Antibody
Evidence has been presented that rabbit homocytotropic antibody prepared against ovalbumin bears allotypic markers of both group a (heavy chain) and group b (light chain). This was shown by specific removal of activity in passive cutaneous anaphylaxis assays by anti-allotype immunoadsorbents. The homocytotropic antibody has properties which indicate that it belongs to a new class of rabbit immu...
متن کاملAntibody Repertoire of Atlantic Salmon: Ig-sec or High-throughput Sequencing of the Variable Region of Igm Heavy Chain
Immunoglobulins (Ig) play a key role in protection of fish against bacterial and viral pathogens. Atlantic salmon has three types of Ig (M, D and T), of which IgM is the main actor of the adaptive humoral immunity. Ig heavy chains are encoded with two loci and their variable regions (VR) are produced by somatic recombination of J, D and V genes. While immunological methods detect specific antib...
متن کاملCOPII vesicles derived from mammalian endoplasmic reticulum microsomes recruit COPI
ER to Golgi transport requires the function of two distinct vesicle coat complexes, termed COPI (coatomer) and COPII, whose assembly is regulated by the small GTPases ADP-ribosylation factor 1 (ARF1) and Sar1, respectively. To address their individual roles in transport, we have developed a new assay using mammalian microsomes that reconstitute the formation of ER-derived vesicular carriers. Ve...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genome Medicine
سال: 2018
ISSN: 1756-994X
DOI: 10.1186/s13073-018-0542-5